In this project, anaerobic ammonia oxidation (Anammox) coupling microbiology mechanism will be investigated in the mangrove wetlands, by in-situ sampling study and microcosm experiment. Therefore, the Anammox rate and contribution under different environmental conditions (pH, substrate concentration, oxidation reduction potential, sulfide, dry-wet alternation, etc.) will be measured. The abundance and distribution of functional genes (Anammox) will be quantified by real-time fluorescent quantitative PCR through the selected molecular markers. To evaluate the key members of Anammox bacteria, clone sequencing will be conducted using the DNA extracted from in-situ and microcosm experiment, and then, the clone libraries will be constructed to compare the community structure characteristics. In addition, microcosm experiment will be stimulated to explore the pattern of Anammox affected by environmental factors. Since the knowledge gap of Anammox in mangrove wetlands, this project can draw a picture of Anammox process in the selected sites. In conclusion, our research can provide direct evidence for the occurrence of Anammox process and contribution in mangrove wetlands, and provide the theoretical basis and data support for wetland nitrogen cycle.
