Human health and biological safety problems resulted from urban drinking water pipe network biofilms pollution have attracted wide concern. The formation of biofilms and its influence on water quantity is closely related to water-biofilms-pipe wall surface. The complex interaction between pollutants and interface is the core of the environmental pollution. Combined with the actual sampling and indoor simulation experiment, this project will use synchrotron radiation based microstructural analysis and modern biotechnology to explore the process of biofilms formation on water-pipe wall micro-surface. In situ test will be carried out from the adhesion of bacteria to biofilms formation. The relationship between environmental factors and elements distribution characteristics of biofilm in microscopic scale will be studied. Bacterial synergy involved in the pipeline corrosion will be discussed. This project will provide the theory basis for the drinking water pipe network biofilms pollution control.