Nanosilver is one of the most widely used nanomaterials due to its unique properties. The wide application of nanosilver-containing products has inevitably led to release vast quantities of nanosilver into the environment, which would posed threats to biosafety and ecological health. The ecological environmental risks of nanosilver have cause wide public concern. However, the recent researches about environmental risks of nanosilver mainly focus on the aerobic environments. The environmental risks under the anaerobic environment have not been systematically investigated. Therefore, the project would stress the environmental behaviors of nanosilver under the anaerobic environment. In the project, the anaerobic environmental behaviors of nanosilver would be investigated firstly and then the effects of natural organic matters would be taken into consideration. Then, the Shewanella sp. would be chosen as the research object to conduct the effects of anaerobic metabolism progresses on the environmental behaviors of nanosilver. Finally, on the basis of the above experiments the roles of quionoe respiration mediated by natural organic matters in the environmental behaviors of nanosilver would be explored and the relationships among nanosilver, microbe and humic substances would be illustrated. Furthermore, by combination with the test results of the microbial growth metabolism the effect mechanisms of quinone respiration mediated by natural organic matters on the environmental behaviors of nanosilver would be revealed. This project would be helpful to deepen the cognition about the problems of environmental behaviors of nanosilver.