Perfluorooctane sulfonate (PFOS) is a persistent organic contaminant and has been widely detected in the sea water. However, toxic effects of PFOS on cardiac development in marine organisms have not been reported. In the present study, we investigated the toxicity of PFOS on the cardiac development using Oryzias melastigma embryos. The embryos at 2 days post-fertilization (dpf) were continuous exposed to PFOS (1, 4 and 16 mg/L) for various periods, cardiac function and morphology were examined at different developmental stages. The results showed that exposure to 4 and 16 mg/L PFOS resulted in enlarged the sinus venosus (SV)–bulbus arteriosus (BA) distance and altered the heart rate. We further investigated eight heart-development related genes to test the effects of PFOS on molecular level. Seven genes were first cloned in O. melastigma and their temporal expression patterns were assayed. Most of

the genes were highly expressed in the 6dpf, which is the critical stage for heart development. Their expression levels upon PFOS exposure were studied. The expressions of GATA4 and NKX2.5 were significantly

down-regulated while COX-2, FGF8 and ATPase were significantly up-regulated at 6dpf. Our results showed for the first time that PFOS exposure affected the expression of cardiac development-related

genes, development and function of heart in the marine medaka.

PFOS Oryzias melastigma Cardiac toxicity Heart-related genes